Use of monoclonal antibodies in an enzyme immunoassay for factor VIII-related antigen.
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منابع مشابه
Use of monoclonal antibodies in an enzyme immunoassay for factor VIII-related antigen.
Two monoclonal antibodies (MAb 53, MAb D7) were produced, each having specificity for Factor VIII-related antigen (FVIIIR:Ag), but exhibiting no inhibitory effect on either procoagulant activity or the ability of von Willebrand factor to agglutinate platelets in the presence of the antibiotic ristocetin. For quantification of FVIIIR:Ag, we used the antibodies in a competitive enzyme-linked immu...
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A sandwich enzyme immunoassay for plasma factor VIII related antigen has been developed which exploits a para-iodophenol enhanced chemiluminescent reaction to detect the horseradish peroxidase label. The assay entailed 15 min incubations with sample and with conjugate and had a detection limit of 0.12 mU. It showed good within batch precision (coefficient of variation = 2.95-5.8%) and results o...
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To the Editor: the capacity of the inhibitor to neutralize FV1II:C activity and may not recognize nonactivating antibodies. However these I g s may sigIn previously reported studies, factor VI11 (FVIII) inhibitors are nificantly influence plasma clearance, survival times, and circulatquantitatively determined by laboratory mixing tests that measure ing levels of infused FVII1:C protein. Both th...
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Various organs, including liver, spleen, heart, lung, kidney, intestines, lymph nodes, pancreas, bone marrow, and thymus, were investigated for the presence of factor VIII-procoagulant antigen (VIIICAg) and factor VIII-related antigen (VIIIRAg), using a panel of monoclonal antibodies directed to factor VIII-von Willebrand factor in combination with a sensitive immunoperoxidase staining techniqu...
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ژورنال
عنوان ژورنال: Clinical Chemistry
سال: 1984
ISSN: 0009-9147,1530-8561
DOI: 10.1093/clinchem/30.1.87